Hepatitis C RNA
Hepatitis virus (HCV) is a small, enveloped, single-stranded positive-strand RNA virus belonging to the Flaviviridae family of the Flaviviridae family. The HCV genome is a long open reading frame (ORF) with non-coding regions on both sides of 5' and 3'. Starting from the 5' end, the coding region consists of 7 gene regions, namely C, E1. E2, NS1, NS2, NS3, NS4 and NS5, the C region encodes a nucleocapsid protein, and the E1 region and the E2/NS1 region encode an envelope protein. The envelope region gene is the largest part of the genomic variation. Therefore, almost no HCV-infected neutralizing antibody may be associated with HCV-infected patients, or it may be a cause of persistent HCV infection and chronic disease. NS2 to NS5 encode non-structural proteins, some of which are not well understood. NS3 encodes a helicase and a protease, and the NS3 protein is highly antigenic, and the produced antibody has important diagnostic value. NS5 encodes an RNA-dependent RNA polymerase, and NS5 protein antibody positivity appears to be associated with viral activity. Laboratory testing of HCV can detect viral RNA by PCR and can also detect antibodies.Basic Information
Specialist classification: Infectious disease examination and classification: blood examination
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
Negative means normal and not infected with hepatitis C virus.
A positive indication is infected with the hepatitis C virus.
ELISA method: negative.
RIA method: negative.
MEIA method: negative S/CO < 1.00; S/N < 2.00. Positive S/CO 0.8 to 1.0; S/N 1.6 to 2.0.
Note: MEIA method gray area: S / CO ≥ 1.00; S / N ≥ 2.0: gray area value of patients should be observed dynamically.Clinical significance
Anti-HCV is a non-neutralizing antibody that is a marker of HCV infection, and the presence of anti-HCV is generally indicative of infectivity. High titers of anti-HCV positive are often associated with existing HCV infections. If HCV-RNA is detected simultaneously, the virus can be diagnosed and contagious.
In the acute phase of the disease, antibodies usually rise after 3 to 6 months after the onset of hepatitis C and exposure to the virus. The increase in antibody can be consistent with or subsequent to the transaminase peak. Anti-HCV generally persists in patients whose infection develops chronically. In cases of remission, antibodies disappear within 6 to 12 months, however, anti-HCV may also be detected for up to four years. Anti-HCV testing can be used for hepatitis C pathogen diagnosis, epidemiological investigation, screening of blood donors and blood products.Positive results may be diseases: hepatitis C virus, post-transfusion hepatitis precautions
HCV-RNA positive, and ALT, AST values are normal, HCV-RNA tracking should be performed regularly to ensure the accuracy of the test results.Inspection process
(1) ELISA method: Indirect method, that is, the recombinant polypeptide antigen on the coated plate first binds to anti-HCV in the serum to be tested, and then binds to the enzyme-labeled anti-human IgG to form HCVAg-anti-HCV- The anti-human IgG complex was enzyme-labeled, and the substrate was reacted to develop color positive. If the serum to be tested has no anti-HCV, it will not develop color and be negative.
(2) RIA method: also an indirect method, which is basically the same as the ELISA indirect method, except that the antibody is labeled with 125I, and the cpm value is finally counted to determine the anti-HCV content.
(3) Microparticle enzyme exemption (MEIA): It is also an indirect method, but it is coated on the microparticles. The labeled enzyme is alkaline phosphatase, and the substrate is 4-methylphosphatidyl ketone. The content of anti-HCV was determined. Works on Axsym instruments, accurate and reliable.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
Generally no complications and harm.