Herpes simplex virus antibody
HSV is a member of the herpesvirus family and is divided into HSV-I and HSV-II. Direct intimate contact and sexual contact are the main routes of transmission and can also be transmitted by droplets and vertical. Named after the blister-like rash often occurs in the acute phase of infection. Clinically, neutralization test, complement fixation test, ELISA, etc. are often used to assist diagnosis. Early diagnosis is valuable for timely treatment.Basic Information
Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection
Applicable gender: whether men and women apply fasting: fastingAnalysis results:
The normal result is negative.
A positive indication is infected with herpes simplex virus.
The normal result is negative.Clinical significance
HSV mainly causes herpetic stomatitis, eczema herpes, herpetic keratoconjunctivitis, herpetic meningitis, neonatal herpes, herpetic vulvovaginitis and the like. Infections outside the reproductive organs were mostly caused by HSV-I (95%), while HSV infection in the reproductive organs was mainly caused by HSV-II (78%). This test does not distinguish between HSV-I and II. Specific IgM antibody-positive or specific IgG antibody titers increased 4-fold, suggesting a recent HSV infection.Positive results may be diseases: oral herpes simplex, herpetic stomatitis precautions
1. In the examination: If used for the diagnosis of acute infection, double serum should be taken in the acute phase and the recovery phase, and IgG and IgM in the serum should be detected at the same time.
2. The same antigenic tablet is used in China, and the fluorescent antibody is replaced by an enzyme-labeled anti-human IgM antibody, and the same effect is obtained by performing enzyme immunolocalization assay.
There are two methods for using ELISA:
(1) The anti-EHF virus monoclonal antibody is combined with the carrier to form a solid phase for capturing a specific antigen, and after adding the serum to be tested, the enzyme-labeled anti-human μ chain is recognized and the substrate is colored.
(2) The anti-human μ chain plate is used to capture IgM in the serum to be tested, and then the EHF virus antigen is added, and finally the anti-EHF virus monoclonal antibody conjugate is labeled with an enzyme, and the substrate is colored.
The antigen used in the ELISA was prepared from the brain tissue suspension of the suckling mouse infected with EHF virus, and the remaining reagents and procedures were the same as the general ELISA.Inspection process
Commonly used in antibody detection methods include complement binding assay, neutralization assay, immunofluorescence and enzyme-linked immunosorbent assay, etc., which are widely used in the diagnosis of acute infections and organ transplant patients, as well as epidemiological investigations. For the diagnosis of acute infection, both serum in the acute phase and recovery phase should be taken, and IgG and IgM in the serum should be detected simultaneously.Not suitable for the crowd
Those who do not have an indication for examination should not do this check.Adverse reactions and risks
Generally no complications and harm.