Mycoplasma pneumoniae cold agglutination test

The most common mycoplasma for human disease is Mycoplasma pneumoniae, which is currently considered to be the pathogen of atypical pneumonia. Isolation of pathogens takes time and the positive rate is low. Currently, immunological detection methods are commonly used.

Basic Information

Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection

Applicable gender: whether men and women apply fasting: fasting

Tips: Check the fasting within 8 hours before the check. Normal value

The serum condensed collectin titer is within 1:32.

Clinical significance

Abnormal results:

Normal human serum contains a small amount of cold agglutinin, about 1% of people can reach 1:32. 75% of patients with mycoplasmal pneumonia, the second week after the disease, the concentration of cold agglutinin can reach 1:32 ~ 1:64 or higher It reached its peak in 4 weeks and fell or disappeared after 6 weeks. A double serum serum titer of more than 4 times or a titer of ≥ 1:64 is diagnostic. Some diseases such as infectious mononucleosis, severe anemia, malaria, myeloma, tropical eosinophilia, mumps-induced orchitis, spirochete, Raynaud's disease, trypanosomiasis, cirrhosis, etc. Positive. Individual agglutination titers can reach 1:32 or above, so the positive test must be combined with clinical analysis.

Need to check the crowd:

Patients with symptoms of pneumonia such as cough and fever are required to perform this test.

High results may be diseases: mycoplasma pneumonia, SARS precautions

Requirements for inspection:

Dizziness occurs during blood tests, and you need to tell your doctor when you want to vomit.

Preparation before inspection:

Fasting within 8 hours before the test.

Not suitable for people:

There is no inappropriate crowd.

Inspection process

The cell membrane component of Mycoplasma pneumoniae (Mac strain) is adsorbed as an antigen on the surface of gelatin particles to prepare sensitized particles, and serially diluted serum samples are added to sensitized particles, such as anti-Pneumococcal antibody in serum samples, which can be combined with sensitized particles. The antigen binds and agglutination occurs after standing; on the contrary, no agglutination occurs.

Not suitable for the crowd

No taboos.

Adverse reactions and risks

Generally no complications and harm.