Parainluenza virus (PIV) is an important pathogen that mainly causes upper respiratory tract infections and severe lower respiratory tract infections in infants and children. Its structure is a single-negative RNA virus, there is a capsule, there are two kinds of spikes on the capsule, namely HN protein and F protein. The HN protein has hemagglutination and neuraminidase activity, and the F protein is similar to the respiratory syncytial virus, mediating the envelope of the virus and the cell membrane fusion of the host.

Basic Information

Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
1. Hemagglutination test, neutralization test, ELISA method: negative. 2. The titer of a single serum neutralizing antibody is within 1:160.

Positive:
Positive (or titer greater than or equal to 1:160): Viral influenza.

Tips: The day before the check, you need an empty stomach. Normal value

Hemagglutination test, neutralization test, and ELISA were negative.

A single serum neutralizing antibody titer ≤ 1:160.

Clinical significance

Abnormal results:

Positive (or titer ≥ 1:160) viral influenza.

Need to check the crowd:

People with flu symptoms, people who are often exposed to public places.

Positive results may be diseases: viral influenza, influenza precautions

Pre-inspection requirements:

(1) The enzyme-linked immunosorbent assay is a highly sensitive test method with many influencing factors. In order to ensure the stability and repeatability of the test results, the concentration of the antigen, antibody and enzyme conjugate should be pre-tested to select the appropriate working concentration in order to control the test conditions.

(2) An empty stomach is required on the day before the examination.

Requirements for inspection:

(1) Note that various controls (including antigens, antibodies, and enzyme conjugate controls, etc., are used in order to compare results).

(2) Double serum must be tested simultaneously on the same plate.

Not suitable for people:

Those who do not have an indication for examination should not do this check.

Inspection process

Clean blood tube is taken from the fasting vein of the test tube, and the tissue is cultured: the virus in the cell is isolated and identified, or the virus present in the respiratory secretion is directly detected, and immunofluorescence assay, PCR, enzyme-linked immunosorbent assay, and the like are used. A significant increase in IgG-specific antibodies in two serum samples collected at appropriate times or detection of specific antibody IgM in a single serum sample led to conclusions.

Not suitable for the crowd

Those who do not have an indication for examination should not do this check.

Adverse reactions and risks

Generally no complications and harm.