Respiratory syncytial virus (RSV) is the most common virus causing lower respiratory tract disease in infants and young children. Respiratory syncytial virus is a single-stranded negative-strand RNA virus that is spherical, has a double-layered lipid vesicle, and has a spike on the capsular membrane, namely G and F proteins. G has an adsorption effect on host cells, and F is a fusion protein. Respiratory syncytial virus (RSV) belongs to the genus Pneumovirus of the Paramyxoviridae family. It has a complex structure and is the most important pathogen of infants and young children with lower respiratory tract infections in the world, especially infants with viral pneumonia and bronchiolitis. The prevention and control measures, so it is of great significance to study the relationship between structure and function in depth. The use of monoclonal antibody (McAb) technology has greatly facilitated the understanding of RSV granule proteins.

Basic Information

Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection

Applicable gender: whether men and women apply fasting: fasting

Analysis results:

Below normal:

Normal value:
no

Above normal:

negative:
The normal value is negative.

Positive:
Positive indicates respiratory viral infection (synaptic viral pneumonia, syncytial viral bronchiolitis).

Tips: Pre-test the concentration of antigen, antibody and enzyme conjugate before testing. Normal value

The normal value is negative.

Clinical significance

Abnormal results:

Positive for respiratory viral infection (synaptic viral pneumonia, syncytial viral bronchiolitis).

People who need to check:

A fever with other symptoms, such as chills, headache, loss of appetite, physical pain or weakness.

Positive results may be diseases: pediatric bronchiolitis considerations

Before inspection:

Enzyme-linked immunosorbent assay (ELISA) is a highly sensitive test method with many influencing factors. In order to ensure the stability and repeatability of the test results, the concentration of the antigen, antibody and enzyme conjugate should be pre-tested to select the appropriate working concentration in order to control the test conditions.

When checking:

1. Pay attention to various controls (including antigens, antibodies, and enzyme conjugate controls, etc., in order to compare results).

2. Double serum must be tested simultaneously on the same plate.

Unsuitable for people: those who do not have an indication for examination should not do this check.

Inspection process

According to the sequence of the N-transcript of the bovine respiratory syncytial virus nucleocapsid protein N gene published on GenBankTM, a pair of specific primers were designed and synthesized, and the target fragment of 596 bp was amplified. The specificity test, sensitivity test and repetitive test were established. RT-PCR method for detection of bovine respiratory syncytial virus. The established RT-PCR method for bovine respiratory syncytial virus has no cross between bovine adenovirus, bovine parainfluenza virus, bovine infectious rhinotracheitis virus and bovine no slurry. The sensitivity of the method is up to 1TCID50. The results show that the method is rapid, sensitive, specific and reproducible, and can be used as a method for detection of bovine respiratory syncytial virus.

Not suitable for the crowd

Those who do not have an indication for examination should not do this check.

Adverse reactions and risks

Generally no complications and harm.